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What settings did you have on your camera for those photos?
Manual mode F11, 1/250, and ISO 100. For most of my macro work I'm either shooting at those settings or they are my starting point.
What magnification level are those photos at?
The fly is at 3x, the ants are at 2, 3, and 5x -or close to it. I've been using magnification as a compositional tool so a lot of the time I don't know what the mag is (unless I happen to look at the lens after the critter takes off and make a mental note of where I had the lens).
Did you use a focusing rail? If so, what is the one you use?
No rail, no tripod -it's too slow for the critters I shoot. Everything is hand held. I just look for ways to brace the rig and I get the flash as close to the critter as possible so I can take advantage of an extremely short flash duration to freeze the motion. My style of macro is very similar to stop motion photography.