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tkhasawinah wrote:
I'm also interested in learning how to use my D800E with a microscope. What kind of microscope and accessories do you need to get results similar to the winners of the 2013 Nikon Small World Photomicrography Competition? Here's the link to their photos:
http://www.nikonsmallworld.com/galleries/photo/2013-photomicrography-competition
I'm sure that there's a myriad choice of techniques and gear but what do you recommend in terms of gear to achieve results at that level? Thanks.
Can't speak for the rest of the images, as I'm not in the field, but looking at 5th and 17th place I can give you a good guess. 17th place is a neuron in the V1 cortex (in a thin slice of brain tissue), which has been filled with a fluorescent dye via the patch electrode (the bright glass thing on the bottom right), and then has been imaged on epifluorescent scope with a 40x lens on a CCD camera (or maybe even an SLR). This setup will run you ~$6k for the lens, $5k for the light, and between $15 and $35k for the scope. 5th place is an immunocytochemical image, where a mossy cell in the hippocampus was tagged for some protein on receptors. The image was made on a Zeiss confocal microscope (~$150k system) or, more likely, on a two-photon scope (>$200k) with a 63x lens.
The point here is that most of those images were done by scientists who (like me) have access to systems that costs tens or hundreds of thousands of dollars. It's pretty difficult to get anything similar using inexpensive scopes and an SLR. Some of the larger things (the snail larvae or the chameleon ) might be visualized with a stereoscope, which runs between $1k-$3k, though you'd still be looking at additional adapters.
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