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there,s no noise in these images.i use nik Define,plus at ISO 100 or even 200 you get no noise on the 5dMK111.
I sharpen my tiffs when done in P/S ,and then the jpegs when resizing for the webb.have a lot of plug-ins and software at my disposal.
think what brian is referring to,is with the mpe,say at f/16,and you were to increase the mag to x5,you would be effectively at f/96. so you need to open the aperture up accordingly to avoid diffraction.so at x5 on full frame f/5.6,or maybe f/6.3 max.for my stacks I usually shoot at f/4.
my problem occurs in the heat of the moment while shooting an insect at low mag,and then increasing,i sometimes forget to open up the aperture accordingly.