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Traditionally with Canon, Mode 1 is used when hand holding steady, and Mode 2 is used when panning (horizontal motion). I do not always turn IS off when shooting my 70-200/2.8L II on a tripod. Being as the purpose of image stabilization is to reduce the effect of vibration, there are other techniques to reduce vibration if you turn IS OFF
I hang a sand bag from the hook under my tripod. This helps damp movement.
In "Proper Long Lens Technique", wildlife photographer Moose Peterson advises:
"First and foremost -you must rest your hand on the lens barrel above the
tripod head! (you can also put your hand on the bottom of the lens barrel if you're
pointing the lens down) You rest your hand on the lens just like you'd rest it in your
lap. Vibrations are what causes images to be out of focus, vibrations that normally
start at the camera. These vibrations travel as a wave to the front element and if
they are not stopped, they travel back through the lens to the film plane, causing
images to be out of focus. The simple resting of a hand on the lens barrel stops this
wave before it reaches the front. No, a bean bag resting in the place of the hand is
not a good idea for many reasons.
Second - use an eyecup and press you eye against it! This again is another way
to minimize or eliminate any vibration that might start with the taking of the
photograph. If you can't remember to press against the eyecup like I did in the
beginning, put a sticky note on the back of the camera to remind you.
Third - roll you finger when firing! Too many photographers poke at their shutter
release to fire the camera, causing all sorts of unwanted movement. You want to
rest your finger on the shutter release at all times and then slightly roll it unto the
shutter release to actually fire the camera. This takes practice, but makes a big
difference!"
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