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This may be one of the craziest questions you ever read.. but,
Recently I got a black, grey, white target to set white balance.
When I use my 2 alien bees 800s, canon 60d, canon 24-70 2.8.. hand held..
In the past I always got clear, crips photos..
But doing the settings to get the histogram correct with the target... all my photos.. the eyes are not crisp..
Shutter Speed 250
54 mm and others. Even at 70mm.
Does the fstop settings have anything to do with Crispness, sharpness?