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Chris and Lazlo,
I used 1600, because the lighting I was using was a tungsten lamp shot through two diffusers to balance the color temp and soften the light cast on the flower. One of the things I run into with orchids is the reflective nature of their petals, so to help control that, I set up the lighting to counter it. At f/9-f/11, the sweet spot of this macro lens at the focal distance I was using, a boost in ISO was required to get the exposure in the ballpark. I know I could have stopped up quite bit and dropped the ISO, but effective focal range decreases substantially, requiring more shots. I'm on a learning path, so any tips or suggestion are appreciated. I usually shoot flowers at f/22 and ISO 100, when using a regular lens, but then again, I'm usually a few feet away...
Greg, If I understand you correctly you shot this outside and made it look like a inside job? Well done!
Not the case at all Chris. Not sure how that came across but it was shot inside....
Now you got me confused Greg. If you are in a controlled condition why not lower the shutter speed about 3 stops?
You used a tripod, right?